Graphene FET Sensors for Alzheimer’s Disease Protein Biomarker Clusterin Detection

We report on the fabrication and characterisation of graphene field-effect transistor (GFET) biosensors for detection Clusterin, a prominent protein biomarker Alzheimer’s disease (AD). The GFET sensors were fabricated Si/SiO 2 substrate using photolithographic patterning metal lift-off techniques with evaporated chromium sputtered gold contacts. Raman Spectroscopy was performed devices to determine quality graphene. GFETs annealed improve their performance before channels functionalized by immobilising surface linker molecules anti-Clusterin antibodies. Concentration also independently verified absorption spectroscopy highly collimated micro-beam light Diamond B23 beamline. achieved through binding reaction between antibody varying concentrations Clusterin antigen from 1 100 pg/mL, as well specificity tests human chorionic gonadotropin (hCG), glycoprotein risk certain cancers. characterized direct current (DC) 4-probe electrical resistance (4-PER) measurements, which demonstrated limit be ? 300 fg/mL (4 fM). Comparison back-gated Dirac voltage shifts concentration show 4-PER measurements more accurate, at present, point requirement further optimisation processes our next generation sensors. Thus, we have successfully promising set biomarker. developed are entirely generic potential applied variety other applications such Parkinson’s, cancer, cardiovascular.